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      產品分類 > 過敏疾病檢測原料 > Bos d 2 ELISA kit 牛過敏原Bos d 2檢測試劑盒

      Bos d 2 ELISA kit 牛過敏原Bos d 2檢測試劑盒

      產品貨號:EL-BD2
      促銷: 12720.00
      價格: 12720.00

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      kit
      美國Indoor

      ELISA kit for measuring bovine Bos domesticus dander allergen, Bos d 2.

      Antibodies:3D4/mAb1

      Sensitivity:0.1 ng/ml

      Limit of Detection:

      0.4 ng/ml

      Contents:

      Monoclonal antibody 3D4, Bos d 2 Standard, Biotinylated antibody mAb1; sufficient reagents for 10 microtiter plates.

      Storage:Store at 4°C
      Not Provided:

      50mM carbonate/bicarbonate buffer, pH 9.6
      Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
      Bovine serum albumin
      Streptavidin-Peroxidase
      1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

      Notes:

      The Bovine Allergen Standard is recommended for immunoassay calibration purposes only. Not recommended for in-vitro antibody measurements, T cell studies, immunization purposes, or other uses.

      Product Resources:EL-BD2 Certificate of Analysis

      For research and commercial use in vitro: not for human in vivo or therapeutic
      use.

      Certificate of Analysis

      Monoclonal Antibody:3D4

      Vial:Red Cap; 100μl

      Immunogen:Bovine dander allergen

      Isotype:IgG1

      Specificity:

      Binds to an epitope on bovine Bos domesticus dander allergen, Bos d 2.

      Purification:

      Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

      Concentration:

      1.33 mg/ml in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.



      Certificate of Analysis

      Monoclonal Antibody:mAb1

      Vial:Brown Cap; 100μl

      Immunogen:Bovine dander allergen

      Isotype:IgG2b

      Specificity:

      Binds to an epitope on bovine Bos domesticus dander

      allergen, Bos d 2.

      Purification:

      Produced in tissue culture and purified by affinity chromatography using recombinant Protein G. Single heavy and light chain bands on SDS-PAGE.

      Biotinylation:

      Biotinylated and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.



      Certificate of Analysis

      Allergen Standard:Bos d 2 Standard

      Vial:White Cap; 400μl

      Composition:

      Recombinant Bos d 2 allergen was prepared in 0.5% HSA/50% glycerol/PBS, pH 7.4.

      Concentration/Calibration: 500 ng/ml. The concentration of the purified recombinant Bos d 2 was determined by amino acid analysis. There are no national or international Reference standards for Bos d 2.

      References:

      1.  Yl?nen J, Virtanen T, Rytk?nen M, M?ntyj?rvi R. Affinity purification of the major bovine allergen by a novel monoclonal antibody. J Allergy Clin Immunol. 1994 May;93(5):851-8.


      2.  Yl?nen J, Virtanen T, Rytk?nen M, M?ntyj?rvi R. Quantification of a major bovine allergen by a two-site immunometric assay based on monoclonal antibodies. Allergy. 1994 Oct;49(9):707-12.


      3.  Rouvinen J, Rautiainen J, Virtanen T, Zeiler T, Kauppinen J, Taivainen A, M?ntyj?rvi R. Probing the molecular basis of Allergy: Three-dimensional structure of the bovine lipocalin allergen Bos d 2. The Journal of Biological Chemistry 1999;274(4):2337-43.


      4.  Saarelainen SA, Kinnunen TT, Buhot C, N?rv?nen AT, Kauppinen AK, Rytk?nen-Nissinen MA, Maillere B, Virtanen TI. Immunotherapeutic potential of the immunodominant T-cell epitope of lipocalin allergen Bos d 2 and its analogues. Immunology. 2008 Mar;123(3):358-66.


      5.  Filep S, Tsay A, Vailes LD, Gadermaier G, Ferreira F, Matsui EC et al. A multi-allergen standard for immunoassays: CREATE principles applied to eight purified allergens. Allergy. 18 Nov 2011; DOI: 10.1111/j. 1398-9995.2011.02750x.


      6.  Kauppinen A, Per?saari J, Taivainen A, Kinnunen T, Saarelainen S, Rytk?nen-Nissinen M, Jeal H, Jones M, Virtanen T. Association of HLA class II alleles with sensitization to cow dander Bos d 2, an important occupational allergen. Immunobiology. 2012 Jan; 217(1):8-12.

      (For complete reference list see EL-BD2 Certificate of Analysis)


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