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      產品分類 > 過敏疾病檢測原料 > Bla g 2 ELISA kit (EL-BG2) 德國小蠊過敏原Bla g2檢測試劑盒

      Bla g 2 ELISA kit (EL-BG2) 德國小蠊過敏原Bla g2檢測試劑盒

      產品貨號:EL-BG2
      促銷: 12720.00
      價格: 12720.00

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      kit
      美國Indoor

      ELISA kit for measuring Blattella   germanica allergen, Bla g 2.

      Antibodies:

      7C11/pAb

      Sensitivity:

      0.5ng/ml

      Limit of Detection:

      0.5ng/ml

      Assay Performance:

      Inquire

      Contents:

      Monoclonal antibody 7C11, Universal   Allergen Standard, Polyclonal Rabbit anti-Bla g 2; sufficient reagents for 10   microtiter plates.

      Storage:

      Store at 4°C

      Not Provided:

      50mM carbonate/bicarbonate buffer, pH 9.6
        Phosphate buffered saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
        Bovine serum albumin
        Peroxidase conjugated Goat anti. Rabbit IgG
        1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

      Notes:


      Product Resources:

      EL-BG2 Certificate of Analysis

      For research and commercial use in vitro: not for   human in vivo or therapeutic use.


      Certificate of Analysis

      Monoclonal Antibody:

      7C11 (Clone 7C11 C2 A6)

      Vial:

      Red Cap; 100μl

      Immunogen:

      Bla g 2

      Isotype:

      Mouse IgG1

      Specificity:

      Binds to an epitope on cockroach Blattella   germanica allergen, Bla g 2.

      Purification:

      Produced in ascites and purified by ammonium sulphate   precipitation and affinity chromatography using Protein A. Single heavy and   light chain bands on SDS-PAGE.

      Concentration:

      1mg/ml in phosphate buffered saline, pH 7.4, based on   A280 for IgG (1.42=1mg/ml), 0.22μm filtered, preservative free.

       
       

      Certificate of Analysis

      Antibody:

      Rabbit polyclonal antiserum

      Vial:

      Brown Cap; 100μl

      Immunogen:

      Recombinant Bla g 2

      Specificity:

      The antiserum contains IgG antibodies to cockroach Blattella   germanica allergen, Bla g 2 and does not react with Bla g 1.

      Activity:

      Titrated for use in ELISA at 1/1000 dilution. Prepared   in 1%BSA/50% glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.



       
       

      Certificate of Analysis

      Allergen Standard:

      Universal Allergen Standard

      Vial:

      White Cap; 400μl/vial

      Composition:

      A formulation of eight purified natural allergens   prepared in 1% BSA/50% glycerol/PBS, pH 7.4.

      Concentration/Calibration:


      Universal Allergen Standard

      Protein Measurement

      Concentration (ng/ml)

      Der p 1

      Amino-acid analysis

      2500

      Der f 1

      Amino-acid analysis

      2500

      Der p 2

      Amino-acid analysis

      1000

      Fel d 1

      Amino-acid analysis

      1000

      Can f 1

      Amino-acid analysis

      2500

      Rat n 1

      Amino-acid analysis

      1000

      Mus m 1

      Amino-acid analysis

      250

      Bla g 2

      Amino-acid analysis

      2500

      References:


      1.  Pollart SM, Smith TF, Morris E, Gelber LE,   Platts-Mills TAE, Chapman MD. Environmental exposure to cockroach allergens:   analysis with monoclonal Antibody based enzyme immunoassays. J Allergy Clin   Immunol 87:505- 510, 1991.

      2.  Gelber LE, Seltzer LH, Bouzoukis JK, Pollart   SM, Chapman MD, Platts-Mills TAE. Sensitization ad exposure to indoor   allergens as risk factors for asthma among patients presenting to hospital.   Amer Rev Respir Dis 147:573-578, 1993.

      3.  Rosenstreich DL, Eggleston P, Kattan M, Baker   D, Slavin RG, Gergen P, Mitchell H, McNiff-Mortimer K, Lynn H, Ownby D,   Malveaux F. The role of cockroach allergy and exposure to cockroach allergen   in causing morbidity among inner-city children with asthma. New Eng J Med   336:1356-1363, 1997.

      4.  Arruda LK, Vailes LD, Ferriani VP, Santos AB,   Pomés A, Chapman MD. Cockroach allergens and asthma. J Allergy Clin Immunol   107:419-428, 2001.

      5. Pomés A, Vailes LD, Helm RM, Chapman MD. IgE   reactivity of tandem repeats derived from cockroach allergen, Bla g 1. Eur J   Biochem 269:3086-3092, 2002.

      6.  Nalyanya G, Gore JC, Linker HM, Schal C.   German cockroach allergen levels in North Carolina schools: comparison of   integrated pest management and conventional cockroach control. J Med Entomol   46:420-427, 2009.

      (For complete reference list see EL-BG2 Certificate of Analysis)

       


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