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      產品分類 > 過敏疾病檢測原料 > Recombinant Bla g 4 重組德國小蠊過敏原Bla g4

      Recombinant Bla g 4 重組德國小蠊過敏原Bla g4

      產品貨號:RP-BG4-1
      促銷: 8720.00
      價格: 8720.00

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      250ug
      美國Indoor
      Allergen:rBla g 4 (Blattella germanica allergen 4)

      Unit:250μg

      Source:P. pastoris

      Mol. Wt:23kD

      Purification:Purified from Pichia pastoris culture by phenol sepharose and gel filtration chromatography. Purity >95% by SDS-PAGE.

      Concentration:

      See product insert.

      Formulation:

      Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 μm filter.

      Storage:Store at -20oC.

      Notes:(1) rBla g 4 is produced in P. pastoris as a glycosylated allergen with MW slightly higher than that predicted for natural Bla g 4 (21kD). (2) Not tested for endotoxin.

      Genbank:U40767

      PDB:3EBk3EBW, X-ray crystal structures

      Product Resources:Recombinant Bla g 4 Certificate of Analysis

      Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.

      References:


      1. Vailes LD, Kinter MT, Arruda LK, Chapman MD. High level expression of cockroach allergen, Bla g 4 in Pichia pastoris. J Allergy Clin Immunol 1998;101:274-80.

      2. Arruda LK, Vailes LD, Hayden ML, Benjamin DC, Chapman MD. Molecular cloning of cockroach allergen, Bla g 4, identifies ligand binding proteins (or calycins) as a cause of IgE antibody responses. J Biol Chem 1995;270:31196-210.

      3. Arruda LK, Vailes LD, Ferriani VPL, Santos ABR, Pomés A, Chapman MD. The role of cockroach allergens in asthma. J Allergy Clin Immunol 2001;107:419-28.

      4. Fan Y, Gore JC, Redding KO, Vailes LD, Chapman MD, Schal C. Tissue localization and regulation by juvenile hormone of human allergen Bla g 4 from the German cockroach, Blattella germanica (L.). Insect Mol Biol, 2005;14:45-53.

      5. Tan YW, Chan SL, Ong TC, Yit lY, Tiong YS, Chew FT, Sivaraman J, Mok YK. Structures of two major allergens, Bla g 4 and Per a 4, from cockroaches and their IgE binding epitopes. J Biol Chem, 2009;284:3148-3157.


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